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发布于:2018-11-9 09:47:43  访问:21 次 回复:0 篇
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Nistered with BPA at 10 mg/kg body weight. TH = Tualang honey
The ovary relative weight in PC group was significantly higher than CO group. Interestingly, treatment with Tualang honey in BPA-exposed rat (TH group) showed significant reduction in body weight compared to the PC group.BPA-exposed rats cannot prevent the reduction of both hormones levels (Figures 1 and 2).17-Estradiol (E2) and progesterone (P4)Estrous cycleThere were no significant change with regards to both E2 and P4 levels in all experimental groups. However, the E2 level in PC group and TH group were slightly increased compared to the CO group (vehicle-treated). The reduction in the P4 level as seen in the PC group (BPA-treated) was slightly prevented with Tualang honey treatment (Figures 3 and 4).Ovarian follicular developmentTable 2 shows the estrous cycle patterns in all experimental groups. In CO group and THC groups, all rats maintained in normal estrous cycle. In PC group, only 37.5 presented normal estrous cycles and a higher percentage showed persistent diestrous (62.5 ). These estrous cycles pattern were significantly different with both CO group and THC group. However, treatment with Tualang honey in BPA-exposed rats showed higher percentage in normal estrous cycle (62.5 ) and lower in persistent diestrous (37.5 ) compared to PC group but not statistically different.Follicle stimulating hormone (FSH) and luteinizing hormone (LH)Serum FSH and LH levels were significantly reduced in PC group and TH group compared to CO group and THC group. It was also noted that Tualang honey treatment onOvarian morphology analysis was conducted by qualitative and quantitative methods. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25447644 Generally, ovaries from all groups displayed all stages of follicular development. Qualitative histological examination revealed that THC group exhibited healthy ovaries comparable to the ovaries of the negative control group (Figure 5). On the other hand, both the PC group and TH group showed some abnormalities of the ovaries with large antral-like follicles that did not arrive at ovulation and the presence of atretic cystic-like follicles. There was less number of corpus lutea observed. However, the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26552366 degree of abnormalities was more Puromycin (Dihydrochloride) buy apparent in the ovaries of the PC group compared to the TH group. Ovarian follicles at different stages were found in the ovaries of experimental groups. No significant difference was observed in the number of preantral, antral follicles and corpora lutea among all experimental groupsTable 2 Pattern of estrous cycle of rats from day 41 to day-70 in all experimental groupsGroup CO PC TH THC Number of rats with normal cycles ( ) 8 (100 )b 3 (37.5 )a 5 (62.5 ) 8 (100 )baPersistent diestrous ( ) 0b 5 (62.5 )a 3 (37.5 ) 0bThe data were presented as number (percentage). Numbers with different superscripts are significantly different. P < 0.05 versus negative control group (CO) and b P < 0.05 versus positive control group (PC).Nistered with BPA at 10 mg/kg body weight. TH = Tualang honey group administered with Tualang honey at 200 mg/kg body weight + BPA at 10 mg/kg body weight. THC = Tualang honey control group administered with Tualang honey at 200 mg/kg body weight.TH group were slightly higher than CO group. However, the difference was not significant.
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